Biomolecular Resource Facility

Mission

 

To assist investigators by providing access to cutting-edge technology, methodology and expertise in order to accurately identify, characterize and quantify molecular species.

Overview of Services

Molecular Weight Determination: from small molecules to proteins. Multiple options are available MALDI-TOF MS, infusion ESI, or LC-MS. For protein analysis, ammonium salt buffers are preferred and detergents must be avoided.
 
Protein Identification: Proteins can be identified either from solutions or gels by using a protease, typically trypsin, and performing MS or MS/MS. Proteins can be identified as pure, protein complexes, or complex mixtures such as cell extracts.
 
Posttranslational Modification Analysis: PTMs such as phosphorylation, glycosylation, acetylation, etc. can be identified directly or preferably through enrichment strategies. 
 
Protein/Peptide Quantitation: SWATH, Label-free, isobarically labelled (iTRAQ, TMT), or SILAC labeled work flows are all supported.
 
Directed Proteomics: Selected reaction monitoring of peptides or small molecules is supported on our Sciex 6500 Qtrap.
 
Data Analysis: For bioinformatics beyond MASCOT or Sequest output, i.e. pathway analysis, statistical analysis, quantitation.
 
Sample Processing: Capabilities for abundant protein depletion, offline LC fractionation, 1- and 2-D gel electrophoresis, capillary electrophoresis, and PTM enrichment are available.
 
Please inquire for more information about any of the above services.

Leadership

Director of Mass Spectrometry and Core Services
William Russell Ph.D
1.106 Libbie Moody Thompson Basic Science Building
301 University Boulevard
Galveston, TX 77555-3579
Email: bill.russell@utmb.edu
(409) 772-3579
 
 

Personnel

Xuemei Luo, Ph.D
Email: xueluo@utmb.edu
(409) 772-8085
 
Zheng W, Ph.D 
Email: zhewu@utmb.edu
(409) 772-8085
 
Cheryl Lichti, Ph.D
Email: cflichti@utmb.edu
(409) 772-8085
 
Location and hours of operation:
Open 8-5:30 M-F
301 University Boulevard
Galveston, TX 77555-3579
 
UTMB Faculty Oversight:
Mariano Garcia-Blanco MD Ph.D
Alan Brasier MD
Alex Kurosky Ph.D

Contacts

Name Role Phone Email Location
William Russell, Ph.D
Director of Mass Spectrometry and Core Services
 
(409) 772-3579
 
bill.russell@utmb.edu
 
1.106 Libbie Moody Thompson Basic Science Bldg.
 
Xuemei Luo, Ph.D
Personnel
 
(409)772-6338
 
xueluo@utmb.edu
 

 
Zheng Wu, Ph.D
Personnel
 
(409)772-6338
 
zhwu@utmb.edu
 

 
Cheryl Lichti, Ph.D
Personnel
 
(409)772-8085
 
cflichti@utmb.edu
 

 

Services

Name Description
Biofluid Fractionation (GPC ONLY)   
IEF (11cm)   
IgY Depletion (ONLY)   
Imaging (Fuji/Perkin Elmer)   
MS Analysis (Progenesis LC/MS; ProteoIQ, etc.)   
Pathway Analysis (IPA)   
Sample Clean-up (Bio-Rad 2D)   
Sample Clean-up (Spin-membrane after Membrane fractionator)   
Saturation Labeling (BODIPY Fl)/If SNOFlo x 2   
SDS (Criterion -Either BODIPY Fl or SYPRO Ruby)   
Stain (Pro-Q Diamond)   
HPLC 

Priced per sample

 
11 CM Strip 

Priced per strip

 
18O Analysis (Digestion Included)   
2D Training   
Analysis of Images 

Priced per hour
Minimum 30 minutes

 
Analytical 

Priced per sample

 
Bio-Fluid Analysis 

Per sample

 
Blot for Westerns   
Criterion gel (BODIPY Fl Label) 

Priced per gel

 
Criterion gel (Comassie stain) 

Priced per gel

 
Criterion gel (No stain) 

Priced per gel

 
Criterion gel (ProQ-D + Sypro stain) 

Priced per gel

 
Criterion gel (Sypro stain) 

Priced per gel

 
Erdman Cycle 

Per cycle

 
Gel picking 

Priced per spot

 
IEF   
Imaging 

Priced per hour
Minimum charge of 45 minutes

 

 
LC-MALDI (LC-MALDI-TOF) 

Priced per sample

 
LC/MS/MS 

Priced per sample

 
LC/MS/MS (non-proteomic) 

Priced per sample

 
MALDI TOF/TOF 

Priced per spot

 
MALDI-TOF 

Priced per sample

 
MALDI-TOF/TOF 

Priced per sample

 
MS (MALDI-TOF) 

 Priced per sample 

 
MS (nanoLC/MS/MS)  

Priced per sample

 
Pre-fractionation (Membrane IEF)   
Preparative Membrane 

Priced per run (3 fractions)

 
ProPic/Digestion (Plate) 

Priced per plate

 
ProPic/Digestion (Spot) 

Priced per spot

 
Protein Assay 

Priced per sample

 
Protein Hydrolysate Analysis 

per sample

 
Sample Collection Surcharge 

Per separation

 
SDS (Spyro Ruby)   
Semi-Preparative 

Priced per sample

 
Set up fee 

Per sample

 
Trypsin Digest 

Priced per sample

 
Trypsin digestion 

Priced per spot

 
Western blot (Criterion gel) 

Priced per gel

 

Services


Search available services: View: by category alphabetically
Analysis of Images (1)
HPLC separations (3)
Imaging (1)
Isoelectric Focusing (2)
MS analysis of gel spots by robots (3)
Manual gel picking, digestion and HPLC/MS analysis (6)
Mass Spectrometry (4)
Other (3)
Protein Chemistry (2)
Proteomics Discovery (14)
SDS-PAGE (5)
Sequencing (2)
Training (3)

Map



Members at UT Proteomics

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D bill.russell@utmb.edu (409) 772-3579
Core Name Primary Contact Email Phone Number/Ext
UTHSA Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. weintraub@uthscsa.edu (210) 567-4043
Core Name Primary Contact Email Phone Number/Ext
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga jezuniga@mdanderson.org 713-745-1749
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D dhawke@mdanderson.org 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Kathryn Aziz ccsgrppa@mdanderson.org 713-792-5743

University of Texas at Austin

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li Li.Li@uth.tmc.edu 713-500-2456